Harvest Horseshoe Crab Blood

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Horseshoe crabs are an example of creatures that inhabited our planet some 230 million years ago. They have not changed a bit over time. They can be easily recognized by their straight rigid tail and hard shell that reminds of a military helmet.
Horseshoe crabs have hemocyanin and not hemoglobin in their blood that contains the copper. Thus these crabs have blue blood which contains amebocytes used to make Limulus amebocyte lysate that helps to detect bacterial endotoxins.

              NATURE | Crash: A Tale of Two Species | Blue Blood | PBS

Since 2004, the wild population of horseshoe crabs has been on the decline. Scientists already learned that captivity isn’t the be-all end-all solution either. While they aren’t necessarily overfished, their population is definitely vulnerable. And you’ll see how if they’re vulnerable, human health and human lives are definitely vulnerable too.

Not surprisingly, extracting the horseshoe crab blood is a gory process. While horseshoe crabs can be raised in captivity, it isn’t that simple. Ethical issues aside of keeping the crabs in captivity, as to be expected, the quality of the crab’s blood deteriorates in captivity. Researchers solve this issue by catching-bleeding-returning wild horseshoe crabs.

While the crabs live on the seafloor, they mate in shallow waters. It’s the perfect opportunity to capture the wild animals. Once captured, the crabs are shipped to research laboratories, and “the tissue around their hearts is pierced and 30 percent of their blood is drained.” After the blood’s been extracted, the horseshoe crabs are returned to the ocean in order to avoid rebleeding.

According to Huffington Post, researchers also found that bled crabs “are more lethargic and less likely to follow tides.” Behavioral changes aren’t even the worst part. The horseshoe crab’s health can drastically be compromised because lower blood levels and cell counts, coupled with unnatural behaviors, can negatively impact the animal’s ability to ward off infections. One research project found that eight percent of the studied returned bled crabs died upon being returned.

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Horseshoe crab blood is unusual for two reasons. Firstly, it gets its incredible blue color from the copper used to carry oxygen around the crab’s body, in the same way the iron in hemoglobin makes our blood red. Secondly, horseshoe crab blood reacts to the presence of bacterial endotoxins, coagulating around the contamination and trapping it in a gel-like substance. The blood is so sensitive that it will react to a contaminant concentration of one part per trillion. The chemical component of the blood that causes this reaction, coagulan, is isolated from crab blood to be used in what is known as Limulus amebocyte lysate testing or LAL. LAL tests are performed on medical equipment, vaccines and other injectables: if they don’t cause a coagulation reaction, they’re clean.

In order to obtain horseshoe crab blood, around 250,000 live crabs are harvested along the east coast of the U.S. each year. They are transported to one of five companies, cleaned and then set up in racks for around 30 percent of their blood to be drawn. They are returned to the sea a few days after being caught and are returned quite a distance from the harvesting sites to ensure the same crabs aren’t bled repeatedly. It’s estimated that between 10 and 30 percent of the crabs die during or after the process. One study has also shown that the process is so taxing that female crabs travel less frequently to spawning grounds after being bled, thereby slowing down the rate of reproduction. 
Therefore, if you have ever used pharmaceutical drugs, then you probably already have horseshoe crab’s blood on your hands.

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Where (and when) do horseshoe crabs evolve?

The evolution of the horseshoe crab extends back far before the dawn of human civilization, before the dinosaurs, before flowering plants... back to the era in our planet's history when visible life first appeared.
Our Evolution section traces the development of the horseshoe crab from the Paleozoic Era and provides an overview of the changes to our planet's geology, climate, flora and fauna from 540 million years ago to the present. Toward the end of the Cenozoic Era, beginning with the Holocene (11,000 BC to the Present), we look specifically at the evolution and development of the Delaware Bay.
If you've ever had a flu shot, know someone with a pace maker or joint replacement, or have given your pet a rabies vaccination, you owe a debt of gratitude to the horseshoe crab.  Vaccines, injectable drugs, intravenous solutions, and implantable medical devices, both for humans and animals, are quality checked for safety using a test that comes from the blood of horseshoe crabs. 

How does horseshoe crab blood protect the public health? 

Humans can become sick if exposed to bacterial endotoxin. It is especially dangerous if endotoxin enters our blood stream directly, such as from an injectable drug. Given this, drugs and medical devices that come in contact with our blood stream are tested to help assure they do not contain harmful levels of endotoxin.  
Like other animals, the horseshoe crab has an immune and blood coagulation system that protects it against infection.  Inside the horseshoe crab’s blood cell (called the amebocyte) are the protiens of its blood clotting system.  These proteins are released in response to the presence of unwanted organisms like Gram negative bacteria and cause its blood to clot around the injury and bacteria, protecting the animal from further harm.
Endotoxin Testing: LAL and TAL 
Research on horseshoe crabs showed that their blood is very sensitive to endotoxin, which is a component of Gram-negative bacteria like E. coli.  In the 1960s (see timeline), Frederik Bang and Jack Levin developed a test from Limulus polyphemus blood that detected the presence of endotoxin. This test, based on the fact that the blood of the horseshoe crab gels or clots when it comes in contact with endotoxin, was called the Limulus amebocyte lysate (LAL)test and was commercialized in the United States in the 1970s.  In Asia, there is a similar test called TAL which takes its name from an Asian species of crab, Tachypleus tridentatus

The LAL and TAL test methods have advanced since the early days, all with the purpose of helping to make injectable drugs, vaccines, and medical devices safer for humans and animals.  In addition to the use of their blood for an endotoxin test such as the gel clot test, the horseshoe crab's DNA has been used to develop a recombinant test method for endotoxin.  Even as alternatives are being developed that will retire or reduce the use of horseshoe crab blood, we will always be indebted to the horseshoe crab's contribution to our health.
In addition, a version of the LAL test has been clear by FDA as a test to aid in the diagnosis of invasive fungal infections.   This test takes advantage of the fact that the horseshoe crabs blood clotting system responds to (1→3)-β-glucans as well as to endotoxins.   (1→3)-β-glucans are a cell wall component of a wide range of fungi.  A glucan test can indicate the presence (or absence) of a fungal infection within a couple of hours of drawing a patient’s blood sample so that appropriate treatment can begin promptly.  Conventional methods of diagnosis can take days of even weeks to give a definitive result.

Dead, stinky horseshoe crabs raise questions by residents about harvest methods for medical research

Charleston County Councilman Dickie Schweers’ waterfront home in McClellanville looked like a horseshoe crab graveyard last Sunday.

National Seashore Massachusetts
Horseshoe crabs (Limulus polyphemus) are an integral part of the marine ecosystem and significant natural resource in many coastal national park units. As a member of the benthic community, they prey on small benthic fauna, such as bivalves and worms. They are preyed upon by sharks, as well as the federally listed loggerhead sea turtle. Horseshoe crab eggs provide a critical food resource for migrating shorebirds (i.e. red knot) which stop along the Atlantic coastline.

You are likely to see an abundance of horseshoe crabs during their peak spawning season in May and June. Females begin to come ashore to spawn along protected sandy beaches during evening high tides during the full and new moons. Males (1/3 the size of females) attach themselves to females as they approach spawning beaches with a pair of modified claws that look like boxing gloves. You may see more than one male crowding a pair forming a large cluster along the water’s edge. Fertilization of the eggs is external and eggs are laid in nests that are dug in the sand by the female. Females may nest several times in one tidal cycle. Spawning sites within the Cape Cod National Seashore include Pleasant Bay, Nauset Marsh, Great Island, and in recent years, East Harbor.

Horseshoe crabs are commercially harvested for bait for American eel and whelk fisheries, and their blood is used to produce a critical pharmaceutical product, Limulus amebocyte lysate (LAL). LAL is the pharmacological standard used to detect fever-causing bacteria in all injectable drugs (i.e vaccines) and implantable medical devices. Throughout their range, managers are concerned about declines in spawning abundance, possible over-exploitation and unsustainable harvest of horseshoe crabs. In Massachusetts, these concerns have led to stricter fishery regulations and even closures of certain areas to harvest. Harvesting horseshoe crabs within the Cape Cod National Seashore boundary is prohibited.

Cape Cod National Seashore is currently participating in the Cooperative Tagging Program through the US Fish and Wildlife Service. CACO Natural Resource Management staff, with the help of the Cape Cod Horseshoe Crab Conservation Association, are actively tagging adult horseshoe crabs within the East Harbor system in North Truro. Tag and recapture data provides more information about horseshoe crab movement and abundance which is crucial for making effective management decisions.  https://www.nps.gov/caco/learn/nature/horseshoe-crabs.htm

                                           Tagged horseshoe crab
                If you find a horseshoe crab with a tag, please click here or call 1-888-LIMULUS (1-888-546-8587).




Dirty Fishing" Emptying Oceans

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Ever since humans created and used tools for everyday life, destruction and greed was born on this Earth. Those animals who become entangled in nets and traps cannot bite their way through human's unnatural strong materials. When those materials get thrown into the environment and into the ocean, it will forever stay in the water without decay. With these large unnatural webs floating in the water, animals die without meaning. With the shadow of greed in the hearts of human's, it continues to overwhelm the Earth as animals once knew it. Now is the time to stop this meaningless killing. 
〜Keiko Olds〜

"Dirty Fishing" Emptying Oceans, Experts Say

In June, along the shoreline of Mauritania, in northwest Africa, scientists made a gruesome discovery: the carcasses of 230 dolphins, a pilot whale, and 15 endangered hawksbill and leatherback sea turtles.
"Because of the mixture of species found, and the fact that some of them were entangled in sections of fishing nets, it is likely that these animals were killed as bycatch," said Jean-Christophe Vie, deputy coordinator of the World Conservation Union's species program, based in Gland, Switzerland.
Across the world's oceans, large commercial fishing boats haul aboard huge nets and 60-mile (97-kilometer) lines teeming with unwanted creatures—bycatch, sometimes referred to as "bykill" or "dirty fishing." 

Bycatch is a mix of young or low-value fishes, seabirds, marine mammals, and sea turtles, often considered worthless and tossed overboard—dead or dying.
The collateral damage amounts to about 30 million tons (about 27 million tonnes) of sea life each year, says marine ecologist Jane Lubchenco of Oregon State University—about one-third the total global catch. Among the worst offenders are shrimp trawlers, who often discard up to 10 pounds of sea life for each pound of shrimp they catch.
"If a hunter is hunting for elk, he's not killing sparrows, eagles, coyotes, and pronghorn," said Elliott Norse, president of Marine Conservation Biology Institute (MCBI) in Redmond, Washington. "That's different in the sea. We fish blindly—and it's an exceedingly wasteful way of doing things."

                                               Bottlenose Dolphin Rescued From Fishnets

Nets Kill Nearly 1,000 Marine Mammals a Day
By National Geographic

Fishing nets intended for other marine species are killing at-risk species of dolphins and porpoises around the world, according to a report commissioned by the nonprofit World Wildlife Fund-U.S.

Leading marine scientists ranked dolphins and porpoises across the globe for the risk they face from lethal fishing nets. Ten species are included in a list of populations conservationists say require urgent action to prevent further deaths. The report lists the following priority locations and species:

Researchers say most of these species are killed by gillnets. Made of monofilament (single-strand) nylon mesh, gillnets are difficult for dolphins and porpoises to see or detect with their sonar.



Once entangled in netting or its supporting ropes, marine mammals face high risk of drowning. Driftnets and crab nets can also kill the mammals. Nontarget species accidentally caught in fishing equipment are known as bycatch.

"Almost one thousand whales, dolphins, and porpoises die every day in nets and fishing gear," said Karen Baragona, of the WWF species-conservation program.

Last year the U.S. Commission on Ocean Policy identified bycatch as the greatest global threat to cetaceans (whales, dolphins, and porpoises). Marine experts estimate that more than 300,000 cetaceans are killed by fishing gear every year.

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Huge fishing net washed up on Church Cove on the Lizard to be turned into clothes


               Stop the madness... end super trawlers!


           Giant super trawler banned from Australian waters

                Healthy Seas - a journey from waste to wear

Australia condemns killing of dolphins and seals by factory fishing trawler

Petition to ban super trawlers sent to parliament as 95-metre boat nears


The pollution of the environments under water is very clear. Lost fishing nets, lines, hooks and weights are found by divers in large numbers on shipwrecks and reefs but also on the sandy seabed around. 

Ghost fishing is a term that describes what happens when derelict fishing gear ‘continues to fish’ The issue of “ghost fishing” was first brought to the attention of world at the 16th Session of the FAO Committee on Fisheries in April 1985. Following debate at COFI, the FAO Secretariat published an in-depth study of the problem. Derelict fishing gear, sometimes referred to as “ghost gear” or “ghost nets” is any discarded, lost, or abandoned, fishing gear in the environment. This gear continues to fish and trap animals, entangle and potentially kill marine life, smother habitat, and act as a hazard to navigation. Derelict fishing gear, such as nets or traps and pots, is one of the main types of debris impacting the marine environment today. Debris in our oceans, seas and inland waters is very big, so big it will probably never disappear. 


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BY Kamekujiraneko